Standardization of amra - Mangifera indica Linn.

amra :

Mangifera indica Linn.

STANDARDIZATION:

iDENTITY, PURITY AND STRENGTH –
Foreign matter Not more than 1 Per cent, Appendix 2.2.2
Total ash Not more than 3 Per cent, Appendix 2.2.3
Acid-insoluble ash Not more than 0.5 Per cent, Appendix 2.2.4
Alcohol-soluble extractive Not less than 10 Per cent, Appendix 2.2.6
Water-soluble extractive Not less than 10 Per cent, Appendix 2.2.7

T.L.C. –
T.L.C. of the alcoholic extract on Silica gel ‘G’ plate using n-Butanol : Acetic acid: Water (4:1:5) shows under U.V. (366 nm) two fluorescent zones at Rf. 0.62 (yellowish) and 0.92 (blue). On exposure to Iodine vapour five spots appear at Rf. 0.07, 0.29, 0.62, 0.77 and 0.93 (all yellow). On spraying with 5% Methanolic-Sulphuric acid reagent and heating the plate for about ten minutes at 110°C five spots appear at Rf. 0.07 (grey), 0.29 (grey), 0.62 (grey), 0.77 (brown) and 0.93 (brown).


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